polymorphism of bovine lymphocyte antigen drb3.2 in holstein bulls of iran using pcr-rflp
نویسندگان
چکیده
the holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (bola-drb3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp). genomic dna was extracted from bull semen using phenol-chloroform method. a two-step pcr was conducted in order to amplify a 284 base-pair fragment of the target gene. amplicons were digested by rsai, haeiii and bstyi restriction endonuclease enzymes. digested fragments were electrophoresed on 8% polyacrylamide gel and visualized after silver staining. seventeen bola-drb3.2 alleles were identified with frequencies ranging from 1 to 21%. sixteen alleles were similar to those reported previously and one was a new allele which has not been reported before. the frequencies of alleles bola-drb3.2 *3, *8, *10, *11, *12, *13, *15, *16, *21, *22, *23, *24, *28, *51, *iaa, *ibb, *qbb were 2, 9, 2, 14, 1, 2, 4, 10, 1, 14, 5, 21, 6, 6, 1, 1, and 1%, respectively. the seven most frequent alleles (bola-drb3.2 *8, *11, *16, *22, *24, *28, *51) accounted for 80% of alleles in the investigated population. this data indicate that the bola-drb3.2 locus is highly polymorphic in holstein bulls of iran.
منابع مشابه
Polymorphism of Bovine Lymphocyte Antigen DRB3.2 in Holstein Bulls of Iran Using PCR-RFLP
The Holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (BoLA-DRB3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was extracted from bull semen using phenol-chloroform method. A two-step PCR was conducted in order to amplify a 284 base-pair fragment of the target gene. Amplicons were digested by RsaI, HaeIII and BstyI ...
متن کاملAssociation of Bovine Follicular Stimulating Hormone (FSHβ) Gene Polymorphism with Reproduction Traits in Iranian Holstein Bulls
The present study was aimed to examine the association of bovine follicular stimulating hormone gene polymorphism with sperm quality traits including sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh m...
متن کاملPolymorphism and Sequencing of DGAT1 Gene in Iranian Holstein Bulls
Quantitative traits locus for milk production traits has been described on centromeric end of bovine chromosome 14. Reports name the acyl coA: diacylglycerol acyltransferase (DGAT1) gene as a potential candidate gene with dinucleotide substitution (AA to GC) in exon VIII which causes the change of lysine to alanine in amino acid (K232A).The aim of the present study was to estimate the frequency...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 4
شماره 3 2006
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023